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             دكتر مجتبی سنکیان  (PhD ایمونولوژی)             
              عضو هیئت علمی گروه ایمونولوژی و آلرژی 
                                                                                                  
  
 
 
        Email: SankianM@mums.ac.ir

آدرس: مشهد، میدان بوعلی، پژوهشکده بوعلی، مرکز تحقیقات ایمونولوژی، بخش ایمونوبیوشیمی
تلفن: 5-05117112611
نمابر:05117112596

 فعالیتهای تحقیقاتی

University Education:

 

Ph.D. student of immunology  Mashhad University of Medical Sciences  (1379-1384)-Top student

M.Sc. in immunology, Tehran University of Medical Sciences, 1994 (1373-1376)-Top student

B.Sc. in Microbiology, Azad University of Qum, 1990 (1368-1372) -Top student

Diploma of High school, Imam Khomeini, High School, Kashan
 

Thesis:

M.Sc. Supervisor, Mahroo Mirahmadian, Ph.D.

Thesis: Assessment of the complement system in patients with beta-thalassemia major
Ph.D Supervisors; Varasteh A.R, Ph.D., Mahmoudi M. Ph.D.,
Thesis: Molecular Characterization of melon major allergen
 
Special findings:
Submission and registration of   melon profilin allergen as Cuc m 2  to International Union of Immunological Society (IUIS) allergen nomenclature subcommittee. 2003
 
Submission and registration of saffron allergen as Cro S 1 to International Union of Immunological Society (IUIS) allergen nomenclature subcommittee.
 

Sankian, M., Varasteh, A.R., Pazouki N., Mahmoudi M, Submission of six cDNA sequences from melon profilin to Gene Bank, Accession Numbers:

AY879598

AY879597

AY292385

AY292386

AY292387

AY292387
 

Varasteh,A., Sankian,M., Pomes,A. and Chapman,M.D. Submission of cDNA sequence of saffron profilin to Gene Bank, Accession Number:

AY898658

Publications:

 
Published:

 

Sankian, M., Varasteh, a., Mahmoudi, M., Sequence homology: A poor predictive value for profilins cross-reactivity. Clinical and Molecular Allergy- 15 Jun 2005 Accepted

 

Sankian M, Varasteh AR, Esmail N, Moghadam M, Pishnamaz R, Mahmoudi M, Melon allergy and allergenic cross reactivity of melon with other allergens. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 1382;4(6): 330-323

 

Sankian M, Hashemi M., Varasteh AR, Anti-D identification by an enzyme-linked antiglobulin test: A comparison study of two methods. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 1383;7(2): 18-21

 

Tavalai S., Sankian M., Kaghazian H. and Varasteh A. R., Minimizing allergen denaturizing parameters in the preparation of saffron pollen extract, Scientific Journal of Birjand Medical University, 7, 18-21, 2000.

 

Fereidouni.M, Sankian.M, Kermani.T, Shakery.M, Akhlaghi.F, Varasteh A. R., Clinical Pattern of Saffron Pollen Allergy, Scientific Journal of Birjand Medical University, 1382; 10: 25-30

 

Fereidouni M, Sankian M, Varasteh A R., Saffron pollen allergy: A Study in Saffron workers, Scientific Journal of Kerman Medical University. 1383; 12: 7-13.

 

Varasteh A. R, Tavallaie S., Kaghazian H., Sankian M.,. Immunoblot analysis of saffron pollen proteins recognized byhuman IgE, IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 1383;7(4): 257-262

 

A. R. Varasteh, F. Vahedi, M.  Sankian, H. Kaghazian, S. Tavallaie, F. G. Shirazi, M. Arefi, M.  Mahmoudi. Development of an ELISA methods for detection of specific IgG subclasses to saffron pollen allergen in human serum, IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 1384; 8: 231-236

 

Abbaszadegan M.R., Pazouki N., Sankian M., DNA extraction from MDE gel stained with silver nitrate, Protocol Online 2004, www.protocol-online.org

 

Taravati MR, Sadeg Khalili F, Sankian M, Sadaghiani M. Intranasal and subcutaneous immunization techniques for anti-sperm antibody induction and disruption of fertility in female rabbits, URMIA MEDICAL JOURNAL 1382;2(14): 86-79

 

Abbaszadegan MR, Shirdel A, Sorouri J, Khameneh Bagheri A, Gholami B, Gholamin M, Sankian M, Detection of minimal residual disease in CML patient after bone marrow transplantation by RT-PCR. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 1380;3(4): 152-147

 

Submitted:

Sankian, M., Varasteh, a., Mahmoudi, M., Molecular cloning, characterization and expression of a major Cucumis melo allergen, Cuc m 2. Clinical and Experimental Allergy – 23 Jun 2005

 

Sankian, M., Varasteh, a., Mahmoudi, M., Structural and Immunological analysis of intermolecular and intramolecular disulfide bond in the melon profilin, Cuc m 2, International Archived of Allergy- 10 Jun 2005

 

M. Sankian, M. Yousefi, N. Pazouki, M. Mahmoudi, AR. One-Step purification of histidine tagged profilin with high purity and yield using metal precipitation.  (Submitted)
 

Projects:

 

1.      A comparison among preparation methods of saffron pollen extracts, 1379, M

2.      The correlation between invivo and invitro diagnostic tests of saffron pollen allergy, 1379, C

3.      Determination of pHi of saffron pollen allergy, 1380,c

4.      Characterization of physicochemical properties of saffron allergen. 1380,c

5.      cloning and expression of saffron pollen allergen, 1380-1383 C

6.      Evaluation of Persian melon allergens, 1381, c

7.      The preparation of animal model to investigate the allergenicity of Cro S1, 1383,M

8.      Production of Monoclonal antibody against saffron profilin, 1382-1383, M

9.      Evaluation of allergy prevalence in Mashad, 1383 C

10.  Cloning and expression of Bet v1, profilin and chitinase for diagnostic proposes., 1382, C

11.  purification of Monoamine oxidase from bovine serum, 1383,
 

Translations:

 

Translation of Basic concepts of immunology from English to Persian 1376

Translation of Cellular and Molecular Immunology (1994) from English to Persian 1378

Translation of Cellular and Molecular Immunology (2003) from English to Persian 1383
 

Teaching experiences:

Laboratory of Immunology:   in School of medicine, Urumia (1376-1378)
Immunology and Serology:  in School of medicine, Urumia (1376-1378)

Immunology: in School of Gynacology, Urumia and Mashad(1376-1378) and(1382)

 

Technical Expertise:

Protein Analysis Techniques:

Western Blot Analysis, Dot Immunobloting, ELISA,RIA, SRID,DID, Agglutination, Immunoflurescence, protein quantification, Gradient SDS-PAGE, Immunoelectrophoresis, complement assay procedures, Polyclonal Antibody Production.

 

Protein purification Techniques:

Chromatography: affinity, immunoaffinity, Ion Exchange, Gel Filtration (Laboratory and Semi industrial Scale, Precipitation techniques, Ultrafiltration techniques

 

Tissue Culture Techniques:

Basic of cell culture, Hybridoma production

 

Molecular Techniques:

PCR, RT-PCR, Primer design, SSCP, Gel Staining Methods, DNA and RNA purification, T/A cloning, E. coli and Pichia pastoris expression system, PCR Cloning

تاريخ بروز رساني : دوشنبه 13/04/90 ساعت 17:18

Mashhad University of Medical Sciences Contact Details:
Main address: University St., Ghoraishy Building 91375-345 Mashhad, Iran ,
Tel:( 98 511) 841 2081-5 , Fax:( 98 511) 8430249 , E-mail: Info(at)mums.ac.ir Members: - Schools and Hospitals School: Dentistry , School: Health , School: Medicine , School: Nursing & Midwifery , School: Paramedical , School: Pharmacy , School: Traditional Medicine , Hospital: Dr.Sheikh , Hospital: Dr.Shariati , Hospital: Emam Reza , Hospital: Ghaem , Hospital: Hashemi Nezhad , Hospital: Ibn-e-Sina , Hospital: Khatam-al-Anbia , Hospital: Montaserie , Hospital: Ommul-Banin , Hospital: Omid , Hospital: Shahid Kamyab , Hospital: Taleghani , History: Mashhad Faculty of Medicine was officially opened on Nov. 23, 1949 by Dr Zanganeh, the minister of culture. The school started out with 61 students, one associate professor and seven tutors. In 1956, when the school of Arts opened, the two schools merged to form the University of Mashhad. In 1989, the faculties offering Medical Sciences' degrees across the country separated from the Ministry of Culture and Higher Education. New emerging Medical Universities have gone under management of Ministry of Health, Treatment and Medical Education. Thus the original University of Mashhad was divided into two independent universities: "Ferdowsi University of Mashhad" and "Mashhad University of Medical Sciences'. Mashhad University of Medical Sciences operates at present with 8 faculties, 32 hospitals, 179 rural and 147 urban health centers. It covers an enormous area stretching from the north east to the central parts of the country, which makes it the largest university of Medical Sciences providing health care and treatment services. With 597 teaching staff, 1645 MDs with different specialties, 138 dentists, 123 pharmacists and 25, 402 employees, the university provides health care and medical services to the area's large population as well as to over 25 million tourists a year.

مشهد، میدان بوعلی، پژوهشکده بوعلی، مرکز تحقیقات ایمونولوژی تلفن: 5-7112611 0511 نمابر: 7112596 0511
پست الکترونیک: IRC@mums.ac.ir
تمام حقوق مادی و معنوی این سایت مربوط به دانشگاه علوم پزشکی مشهد می باشد و هرگونه کپی برداری از مطالب آن تنها با ذکر منبع بلامانع است.