Sankian, M., Varasteh, A.R., Pazouki N., Mahmoudi M, Submission of six cDNA sequences from melon profilin to Gene Bank, Accession Numbers:
AY879598
AY879597
AY292385
AY292386
AY292387
AY292387
Varasteh,A., Sankian,M., Pomes,A. and Chapman,M.D. Submission of cDNA sequence of saffron profilin to Gene Bank, Accession Number:
AY898658
Publications:
Published:
Sankian, M., Varasteh, a., Mahmoudi, M., Sequence homology: A poor predictive value for profilins cross-reactivity. Clinical and Molecular Allergy- 15 Jun 2005 Accepted
Sankian M, Varasteh AR, Esmail N, Moghadam M, Pishnamaz R, Mahmoudi M, Melon allergy and allergenic cross reactivity of melon with other allergens. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 1382;4(6): 330-323
Sankian M, Hashemi M., Varasteh AR, Anti-D identification by an enzyme-linked antiglobulin test: A comparison study of two methods. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 1383;7(2): 18-21
Tavalai S., Sankian M., Kaghazian H. and Varasteh A. R., Minimizing allergen denaturizing parameters in the preparation of saffron pollen extract, Scientific Journal of Birjand Medical University, 7, 18-21, 2000.
Fereidouni.M, Sankian.M, Kermani.T, Shakery.M, Akhlaghi.F, Varasteh A. R., Clinical Pattern of Saffron Pollen Allergy, Scientific Journal of Birjand Medical University, 1382; 10: 25-30
Fereidouni M, Sankian M, Varasteh A R., Saffron pollen allergy: A Study in Saffron workers, Scientific Journal of Kerman Medical University. 1383; 12: 7-13.
Varasteh A. R, Tavallaie S., Kaghazian H., Sankian M.,. Immunoblot analysis of saffron pollen proteins recognized byhuman IgE, IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 1383;7(4): 257-262
A. R. Varasteh, F. Vahedi, M. Sankian, H. Kaghazian, S. Tavallaie, F. G. Shirazi, M. Arefi, M. Mahmoudi. Development of an ELISA methods for detection of specific IgG subclasses to saffron pollen allergen in human serum, IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 1384; 8: 231-236
Abbaszadegan M.R., Pazouki N., Sankian M., DNA extraction from MDE gel stained with silver nitrate, Protocol Online 2004, www.protocol-online.org
Taravati MR, Sadeg Khalili F, Sankian M, Sadaghiani M. Intranasal and subcutaneous immunization techniques for anti-sperm antibody induction and disruption of fertility in female rabbits, URMIA MEDICAL JOURNAL 1382;2(14): 86-79
Abbaszadegan MR, Shirdel A, Sorouri J, Khameneh Bagheri A, Gholami B, Gholamin M, Sankian M, Detection of minimal residual disease in CML patient after bone marrow transplantation by RT-PCR. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 1380;3(4): 152-147
Submitted:
Sankian, M., Varasteh, a., Mahmoudi, M., Molecular cloning, characterization and expression of a major Cucumis melo allergen, Cuc m 2. Clinical and Experimental Allergy – 23 Jun 2005
Sankian, M., Varasteh, a., Mahmoudi, M., Structural and Immunological analysis of intermolecular and intramolecular disulfide bond in the melon profilin, Cuc m 2, International Archived of Allergy- 10 Jun 2005
M. Sankian, M. Yousefi, N. Pazouki, M. Mahmoudi, AR. One-Step purification of histidine tagged profilin with high purity and yield using metal precipitation. (Submitted)
Projects:
1. A comparison among preparation methods of saffron pollen extracts, 1379, M
2. The correlation between invivo and invitro diagnostic tests of saffron pollen allergy, 1379, C
3. Determination of pHi of saffron pollen allergy, 1380,c
4. Characterization of physicochemical properties of saffron allergen. 1380,c
5. cloning and expression of saffron pollen allergen, 1380-1383 C
6. Evaluation of Persian melon allergens, 1381, c
7. The preparation of animal model to investigate the allergenicity of Cro S1, 1383,M
8. Production of Monoclonal antibody against saffron profilin, 1382-1383, M
9. Evaluation of allergy prevalence in Mashad, 1383 C
10. Cloning and expression of Bet v1, profilin and chitinase for diagnostic proposes., 1382, C
11. purification of Monoamine oxidase from bovine serum, 1383,
Translations:
Translation of Basic concepts of immunology from English to Persian 1376
Translation of Cellular and Molecular Immunology (1994) from English to Persian 1378
Translation of Cellular and Molecular Immunology (2003) from English to Persian 1383
Teaching experiences:
Laboratory of Immunology: in School of medicine, Urumia (1376-1378)
Immunology and Serology: in School of medicine, Urumia (1376-1378)
Immunology: in School of Gynacology, Urumia and Mashad(1376-1378) and(1382)
Technical Expertise:
Protein Analysis Techniques:
Western Blot Analysis, Dot Immunobloting, ELISA,RIA, SRID,DID, Agglutination, Immunoflurescence, protein quantification, Gradient SDS-PAGE, Immunoelectrophoresis, complement assay procedures, Polyclonal Antibody Production.
Protein purification Techniques:
Chromatography: affinity, immunoaffinity, Ion Exchange, Gel Filtration (Laboratory and Semi industrial Scale, Precipitation techniques, Ultrafiltration techniques
Tissue Culture Techniques:
Basic of cell culture, Hybridoma production
Molecular Techniques:
PCR, RT-PCR, Primer design, SSCP, Gel Staining Methods, DNA and RNA purification, T/A cloning, E. coli and Pichia pastoris expression system, PCR Cloning
تاريخ بروز رساني : دوشنبه 13/04/90 ساعت 08:55
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