Head: Dr. Jalil Tavakol Afshari, PhD in Cellular and Molecular Immunology Associate Professor Staff: Dr. Hoshang Rafatpanah, Assistant Professor Mrs Nasrin Moheghi (MSc in Immunology) Mrs Rashin Gangali (MSc in Biotechnology) Mrs Azam Borok (BSc in Biology) Dr. Seyede Sedighe Fatemi, MD

This unit has three laboratories:

Immunogenetics

Cell culture

Immunology

Main research:

• The effects of immune mechanisms and genetic immunoflurescent incidence of diseases (autoimmune, infectious disease and cancer) and common disorders in the Iranian population, especially North-East region
• Promoting new basic and applied research
   

Instruments:

 

• Immunofluorescence microscopy
• cell system Harvester
• Invert Microscope
• spectrophotometer
• Freezer 70
• bath, Shaker, Vortex
• Thermo cycler
• protein purification equipment including: column chromatography  UV and visible
• Gel documentation
• ELISA reader, ELISA washer and incubated plates ELISA
• Power Supply
• Incubator Co2, nitrogen tank
• Centrifugal refrigerator and ordinary
• vertical and horizontal gel electrophoresis tank
• Fractions counter - Laminar Air Flow
• Western Blot 
   

Published Articles:

Kyanifar HR., Tavakkol Afshari, J., Talebi, S. and Brouk, A. D28G mutation in an Iranian girl with congential glucose galactose malabsorption: A case report. In Press. AIM.

Arab, HR., Tavakkol Afshari, J., Radvar, M., Melati, E., Ebadian, AR. Analysis of -1082 interleukin-10 gene polymorphism in Iranian patients with generalized aggressive periodontitis. In Press. Medical Science Monitor.

Ghazvini, K; Ghanaat, J; Tavakkol Afshari, J. and Malvandi M.Prevalence of genital Chlamydia in male patients with urethritis attending STD clinics in Northeast of Iran. In Press. Eastern Mediterranean Health Journal.

Jalil Tavakkol Afshari, Zahra Rezaieyazdi, Farnaz Shoja-Taheri and Majid Sadeghizadeh. The role of HLA-DQB1 alleles in suceptability to Rheumatoid Arthritis. In Press. APLAR Jorunal of Rheumatology.

Jalil Tavakkol Afshari, Reza Farid Hosseini, Sara Hosseini Farahabadi, Farhad Heydarian, Mohammad Hossein Boskabady, Rasoul Khoshnavaz, Alireza Razavi, Ehsan Ghayoor Karimiani, and Gholamreza Ghasemi.Association of the Expression of IL-4 and IL-13 Genes, IL-4 and IgE Serum Levels with Allergic Asthma. Iran J Allergy Asthma Immunol. June 2007; 6(2): 67-72 .

Zamani AR., Sadeghaina S., Tavakkol Afshari J., Nasiri E. And Amiri I. In vitro semi-quantitative determination of human gamma-interferon-mRNA level by PCR. Acta Medica Iranica. 45(1), 8-12, 2007.

Sara Hosseini-Farahabadi, Jalil Tavakkol-Afshari, Houshang Rafatpanah, Reza Farid-Hosseini and Mohammad Khaje Daluei Association between the polymorphisms of IL-4 gene promoter (-590C>T), IL-13 coding region (R130Q) and IL-16 gene promoter (-295T>C) and allergic asthma. IJAAI. 6(1): 9-14, 2007.

Arab, HR., Tavakkol-Afshari, J., Radvar, M., Moeintaghavi, A., Sargolzaei, N., Rigi, A. and Shirkhani, M. IL-1beta +3954 Genetic Polymorphism Association with Generalized Aggressive Periodontitis in Khorasan-Iran Province. J. Med. Sci. 2007; 7(2): 222-227.

Mamouri Gh; Boskabadi H; Tavakkol Afshari j; Naseri F; Shakeri MT. Evalution of quantitative IL-6 in the diagnosis of neontalal sepsis. Medical J. of MUMS. 49(93), 253-265, 2006.

Arab HR., Tavakkol Afshari J, Moeintaghavi A, Sargolzaie N and Brook A. In-vitro evalution of periodontal dressing plus calcium hydroxide on L929 fibroblast cells. J. Dentistry. Mashhad University of Medical Sciences, 2006; 30:279-88.(Persion)

M.A. Hajzadeh , Tavakkol Afshari, J. Ghorbani, A. M.T. Shakeri. Antiproliferative property of aqueous extract of Allium Sativum on Hep2 and L929 cells In Vitro. In press. Journal of Medicinal Plants. 5(15), 2006 (Persian).

Pishdadian A., Tavakkol Afshari, J., Mahmoudi, M., Foroughi Poor, M., Moalem, A., Shahami, SR. and Fahmideh Kar, M. Detection of the soluble form of the Fas/APO-1 receptor in patients with human T-lymphotropic virus type 1-associated myelopathy. J. Iranian Basic Medical Science. 2006; 9(2), 144-152.

Ghoddusi, J., Tavakkol Afshari, J., Bidar M., Khalili, M. Cytotoxic Effect of Amalgam and Mineral Trioxide Aggregate on Human Gingival Fibroblast Cell Line Culture. J. Mashhad Dental School. 2006; 30(1&2), 25-31. (Persian).

Hosseini-Farahabady, S., Tavakkol Afshari, J., Ganjali, R., Rafatpanah, H., Ghaffari, J. and Farid Hosseini, R. Association between the polymorphism of TGF-b1 gene promoter (-590 C>T) and idiopathic chronic urticaria in northeastern Iran. IJAAI. 2006; 5(3): 109-113.

Talebi, S., Tavakkol Afshari, J., Rakhshandeh, H., Feizi, R. and Seifi, B. Antiproliferative Effects of Fresh Red Garlic Extract on Human Transitional Cell Carcinoma in vitro. Int. J. Agri. Biol., 2006; 8(5), 609-614.

Bajestan MN, Radvar M, Afshari J.T., Naseh MR and Arab HR. Interleukin-6 production by cultured peripheral blood monocytes before and after stimulation by E. coli lipopolysaccharide in Iranian patient with aggressive periodontitis. Med Sci Monit, 2006; 12(9): CR393-396.

Sharefe, M., Omidi, H., Tavakkol Afshari, J., Ghanadan, A., Taghepoor, R. and Khodadoost, MA. Study of the relationship between human papillomavirus and laryngeal dysplasia in Mashhad-Iran. The Iranian Journal of Otorhinolaryngology. 2006; 18(1), 9-13.

Tavakkol Afshari, J., Ghoddusi, J., Behforuzi, Eand Kalili, M. Cytotoxic Effect of Amalgam and Mineral Trioxide Aggregate on Mouse Fibroblast Cell Line (L929). J. Mashhad Dental School. Vol. 29 (3&4); 2006, 177-186.

Tavakkol Afshari, J., Rakhshandeh, H., Zamani, AR., Mahdavi Shahri, N., Ghazezadeh, L., Norozi, M. and Vahedi, F. Anti-tumoral activity of whole extract from bitter melon on human Caucasian larynx carcinoma cell line (Hep2). Hakim Research J. Vol. 8, No. 2, 2005; 47-54.

Zamani, AR., Tavakkol Afshari, J. and Alikhani, MY. Molecular cloning and expression of human gamma interferon full cDNA gene in CHO cells. IJI 3(1), Winter 2006.

Arab, H.R.,Tavakkol Afshari, J., Radvar, M., Mointaghavi, A., Sargolzai, N. Hematian, M., Ebadian, AR., Ganiali, R. Association between TGF-β1 gene polymorphism (-509C/T) with aggressive periodontitis. J. Med. Sci. 6(3): 448-452, 2006.

M. Rad, M. Ahmadim R. Farid Hosseini, A.A. Ashkar, and J. Tavakkol Afshari. The role of interleuking-12 in protection induced by CpG ODN against Listeria monocytogenes in BALB/c and C%&BL/6. Comparative Clinical Pathology. Published on line, 1 February 2006.

J. Tavakkol Afshari, M.A. Hajzadeh, A. Ghorbani, H. Parsaie. Ethanolic extract of Allium Sativum has antiproliferative effect on Hep2 and L929 cells. Pharmacognosy Magazine2(5); 29-31, 2006.

M. Radvar, J. Tavakkol Afshari, M. Naghebzadeh, M.R. Naseh and H. Arab. An evaluation of interleukin-6 production by cultured peripheral blood monocytes before and after stimulation by E. coli lipopolyssacharide in aggressive periodontitis from a persian population. J. Iranian Basic Medical Science. Vol. 8, No.3 (27), Autumn 2005.

J. Tavakkol Afshari, N. Ghomian, A. Shameli, M. T. Shakeri, M. A. Fahmidehkar, M. Mahajer, R. Khoshnavaz, and M. Emadzadeh. Determination of Interleukin-6 and Tumor Necrosis Factor-alpha concentrations in Iranian-Khorasanian patients with preeclampsia. BMC Pregnancy and Childbirth 5:14, 2005.

Naderinasab M, Tavakkol Afshari J, Fatehmanesh P, Faramarzi H and khodadost MA. Determination of Resistant Staphylococcus Aureus to Methicillin through Phenotypic Methods and Study of mecA Gene by Multiplex method. Medical j. Mashhad University of Medical Sciences 48(87), 7-16, 2005.

Sadeghian, A., Tavakkol Afshari, J., Ghanaat, J., Sadeghian H., Namaii, MH. and Khodadoost, MA. Comparison of three methods for the identification of Mycobaterium tuberculosis. Int. J. Agri. Biol. 7(2); 203-206, 2005.

Tavakol Afshari, J., Hutchinson, IV., Jorgensen, T.N., Kay, R.A.The immunoregulatory effect of transforming growth-b with a specific T-cell receptor. I.B.J. 9(1); 9-14, 2005.

Tavakkol Afshari, J., Hutchinson, IV. Analysis of IL-4 mRNA level by RT-PCR and association with IgE production in different rat strains, J. Iranian Basic Medical Science. 7(2); 120-124, 2004.

Tavakol Afshari, J., Sadeghian, A. and Khaleli, M. Extraction of lipoplysaccride (LPS) from Escherichia Coli's cell wall and it's evaluation as a mitogen for proliferation of B-lymphocytes by MTT test. Modarres Journal of Medical Sciences 7(1), 39-48, 2004.

Arab, HR., Tavakkol Afshari, J., M., Radvar, M., Khosrave, R. and Khodadoost, MA. Association of HLA-DQB1-0201 with generalized aggressive periodontitis in Khorasan-Iran province. J. Dental School, Shahid Beheshti Medical Sciences University 3(22), 485-487, 2004.

Tavakkol Afshari, J., Farid, R., Enzevaei, A., Jabari, M., Zamani, AR., Khoshvavaz, R., Orouhi, E., Esmaeli, E., Brouk, A. and Fahmedehkar, M. The role of Th1 and Th2 cytokines in immune response to HTLV-I associated myelopathy/tropical spastic paraparesis. Immunology 2004, Medimond S.r.1., 479-482, 2004.

Arab, HR., Tavakkol Afshari, J., Tamiz, M., Radvar, M. and Shirkhani, M. IL-1b⁺³⁹⁵⁴ genetic polymorphism association with generalized aggressive periodontitis in Khorasan-Iran province. Immunology 2004, Medimond S.r.l. 5-8, 2004.

Fazly Bazzaz, BS., Tavakol Afshari, J., Ahmadi, A, Sabeti, Z and Safei, B. HESA-A, its cytotoxicity and mutagenicity. Immunology 2004, Medimond S.r.l., 381-387, 2004.

Tavakol Afshari, J., Gholamzad, M. and Zamani, AR. Purification and characterization of recombinant IL-4 and anti-IL-4 antibody from transfected CHO cell line and hybridoma respectively. J. Iranian Basic Medical Science, 6(3), 194-207, 2003.

Tavakol Afshari, J., Zamani, A.R., Behravan, J., Sheshaeian, B. and Seifi, B. In vitro semi-quantitative determination of human gamma-interferon mRNA level by RT-PCR. Hamadan Journal of Medical Science, 28(2), 5-11, 2003.

Tavakol Afshari, J., Khajeh-Karamodini, M., Khayat-Moghadam, M., Mahdavi-Shahri, N., Zamani, A.R. and Broumand, A.R. Pattern of Helicobacter pylori antigens isolated fron gastric biopsy in patients with peptic ulcer. Hamadan Journal of Medical Science, 9(4), 5-10, 2003.

Torki, M., Arshami, J., Eftekhar Shahroodi, F., Tavakoli Afshar, J. and Golian, A. Effects of dietary n-3 and n-6 polyunsaturated fatty acids on performance and humoral immune response in Broiler chicks. Iranian J. Agric. Sci. 34(1), 115-125, 2003.

Tavakkol Afshari, J., Shahrokh Abadi, KH. And Rakhshandeh, H. Evalution of cytotoxic effect of Safferon on hepatocellular carcinoma cell line. Proceedings of the 3^rd National Symposium on Saffron. 220-226, 2003.

Ravansalar, H, Tavakol Afshari, J, Varasteh, A.R. and Seyedeen, M. Production and purification of polyclonal antibody to human insulin. ASRAR, 2(9), 37-49, 2002.

Hassan-Zadeh Khayat, M., Tavakol Afshari, J. and Shafeei Meybodi, A.M. Determination of the amount of plasma's acetaminophen by spectrophtometery and HPLC. J. Iranian Basic Medical Science. 5(1), 2002.

Majid-Zadeh Heravey, R., Arshami, J. and Tavakol Afshari, J. Effects of energy and protein on growth and humoral immune response in broiler chicks. J. Agriculture Science. 12(1), 2002.

Tavakol Afshari, J., Shirdel, A. and Broumand, A.R. Establishment of HTLV-I infected T cell line from ATL patients in Khorasan provience. Kosar Medical J. 2(7), 2002.

Tavakoli Afshari, J. and Liu, TZ. Rapid spectrophotometric methos for the quantitation of acetaminophen in serum. Analytica Chimica Acta, 443 (1), 165-169, 2001.

Tavakkol Afshari,J., Dastghayb, M., and Ravasoli, A.R. Characterization of electrolyzed and non-electrolysed antigenic subunits of Echinococus Granulosus cyst fluid in hydatid patients. Scandinavian Journal of Immunology, Vol. 54, Supp. 1, 2001.

Tavakkol Afshari, J., Shirdel, A. and Broumand, A.R. Establishment of human T-cell line infected with HTLVI from ATL patients in Khorsan –IRAN. Scandinavian Journal of Immunology, Vol. 54, Supp. 1, 2001.

Bejeh, N., Nazem, F., Tavakol Afshari, J, Nejat-Shokohe, A., Mahmmoudi, M. and Rastein, M. The effect of two eccentric and concentric exercise pattern on given immune system parameters of athletic women. J. Iranian Basic Medical Science. 3(1), 2001.

Beijeh, N., Nazem, F., Tavakol Afshari, J. and Nejat-Shokohe, A. Compersion of inhibitory effect of eccentric and concentric exercise of atheletic women's immune system. Modaress J. of Medical Science. 3(2), 2001.

Tavakol Afshari, J., Hutchinson, IV and Kay, RA. Long – term alloreactive T cell lines and clones express a limited T cell receptor repertoire. Transplant Immunology. 5:122-128, 1997.

Tavakol Afshari, J., Hutchinson, IV., Kay, RA. Limited heterogeneity of TCR Vb gene utilisation by alloreactive T cells. Immunologist. Vol. 54, Suppl. 5, 1995.

Tavakol Afshari, J., Hutchinson , IV and Kay , RA. Common N- region sequences and motifshared by the TCRVB of alloreactive T cells. Immunology. 1995; Suppl.3.

Tavakol Afshari, J., Hutchinson, IV., Kay, RA. Characterization of allospecific T cell-clones. Immunology. 1994:83 Suppl. 1.

Tavakoli Afshari J.,Hutchinson,IV and Kay, RA. Characterization of allospecific T cell-clones. Immunology. 1994:83 Suppl. 10.

Tavakoli, J., Haghi, Z., and Zehtabchi, S. Immunodiagnostic tests in hydatidosis of the lung.M.J.IRI. 6:101-103,1992.

Farid, R., Baradaran, H., Nikbin, B., Tavakol, J. and Amini, H. The distribution of class I HLA. M.J.IRI. 3:43-45, 1989.

Tavakoli, J. and Liu, T. Development of a rapid spectrophotometer screening method quantitation of acetaminophen in human serum. M.J.IRI. 2:243-247, 1988.