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MUMS Electron Microscope Unit
Buali (Avicenna) Research Institute
Electron microscope unit is located at north-west of Avicenna research institute. The unit space is about 140 square meters.
This unit has following facilities:
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The transmission electron microscope is used in both metallurgy and biological sciences. It is a scientific instrument that uses a beam of electron, magnetic lenses focus the electron beam and magnifies the image after the electrons pass through the specimen, thus transmission electron microscope reveals the interior of the specimen.
The specimens must be very thin and be able to withstand the high vacuum present inside the instrument, thin sections of samples are placed on the copper grid and are stained with heavy metals, the slice of tissue can then be studied.
The EM instrument in this unit was bought in 1997 and Dr. Jafari installed it and put in working.
Dr. S. Hassan Alavi has been appointed as head of electron microscope unit since June 2006.
In addition to standard negative film recording, the instrument is equipped with a CCD camera for digital image recording by Dr. Alavi.
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Offer practical efficient services in the field of electron microscopy for scientists and investigators. We hope to be able to improve our services with advances in technology and update our facilities as much as possible such as digital imaging. |
- TEM. Leo910
- Ultra microtome LKB
- Knife maker LKB
- Digital weighter
- Diamond knife
- PH meter
- Magnetic mixer
- Light microscope Olympus
- Light microscope novex
- Stereomicroscope leica
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Oven 50
What is an Electron Microscope... |
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In this unit education courses on principles of transmission electron microscopy is Periodically offered for undergraduate students of Ferdowsi university, and Postgraduate students of anatomical science at MUMS.
In addition electron microscope unit has accomplished workshops on practical Training of electron microscopy many times for applicants such as faculty Members and postgraduate students.
Research projects in this unit has been performed with cooperation of different research Departments or independently, for example: Ultra structure study of spermatogenesis Cells after exposure of morphine in adult male mouse.
The tools and technical services for performing the Thesis of postgraduate students Of MUMS, and other universities has also been done.
| Clinical application of EM: |
Electron microscope has been used in all areas of biological and biomedical Investigations, because of its ability to view the finest cell structures, and It is also used as a diagnostic tool in pathology labs for improving diagnostic and treatment of disease.
Electron microscope unit offers the tools and technical services for research And training for those who do not necessarily want to operate the electron Microscope instrument, or to prepare specimens themselves. The staff of the Electron microscope unit offers training and practical services including Microscope operation, specimen preparation, digital photography and Image analysis.
New services in IMU:
Electron microscpe unit offers technical services in Immunoelectron microscopy, to detect the intracellular location in structures of particular proteins.
| Specimen Preparation for TEM: |
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Procedure:
1. Primary fixation (glutaraldehyde 2-4%)
2. Secondary fixation (osmium tetroxide 1-2%)
3. Dehydration
4. Infiltration
5. Embedding
6. Sectioning
7. Staining |
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Buali Research Institute
